Table 3.
T cell immune dominance of SARS-CoV-2 proteins.
| ID. | ∑ SFU | ORF3a | N | Nsp12 | Nsp5 | S (A & B) | S-RBD | M |
|---|---|---|---|---|---|---|---|---|
| dC1 | 191 | 10% | 12% | 13% | 0% | 40% | 7% | 18% |
| dC2 | 267 | 12% | 25% | 12% | 0% | 40% | 2% | 10% |
| dC3 | 230 | 10% | 13% | 3% | 5% | 50% | 10% | 10% |
| dC4 | 264 | 3% | 21% | 6% | 3% | 47% | 7% | 13% |
| dC5 | 216 | 1% | 16% | 0% | 2% | 49% | 23% | 9% |
| dC6 | 332 | 5% | 31% | 11% | 2% | 35% | 4% | 14% |
| dC7 | 253 | 12% | 10% | 9% | 1% | 51% | 3% | 15% |
| dC8 | 184 | 6% | 21% | 17% | 1% | 31% | 16% | 7% |
| dC9 | 81 | 0% | 19% | 0% | 0% | 24% | 8% | 49% |
| na | 6% | 18% | 8% | 2% | 40% | 9% | 16% | |
| σ | na | 4% | 6% | 6% | 3% | 9% | 6% | 12% |
| # Pept. | na | 66 | 102 | 231 | 74 | 315 | 53 | 53 |
| /(# Pept.) | na | 0.09% | 0.18% | 0.03% | 0.03% | 0.13% | 0.17% | 0.30% |
The total SARS-CoV-2-specific T cell mass (∑SFU) was calculated by adding up for each SARS-CoV-2-recovered donor the numbers of SFU elicited by all SARS-CoV-2 peptide pools in that donor at 1.5 µg/mL (see the raw data in Supplementary Table 5 ). In the top panel, the percentage of T cells targeting each of the SARS-CoV-2 antigens is shown relative to the total clonal SARS-CoV-2-specific T cell mass in that individual, representing an immune dominance index. The superimposed heatmap specifies the affinity level of the respective T cell population, with the color code defined in Table 2 . The lower panel shows the mean percentage () and SD of this immunodominance index for the cohort. Addressing the hypothesis that T cell immune dominance of a SARS-CoV-2 antigen is related to its size, the number of peptides in each pool is shown (# Pept.) and the mean immune dominance index ( ) is normalized for the number of peptides ( /(# Pept).