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. 2021 Mar 2;31(7):3194–3212. doi: 10.1093/cercor/bhaa414

Figure 4.

Figure 4

Shox2 KO decreases the ratio of cells with spontaneous action potentials in the anterior paraventricular thalamus. A. Example traces of attached-cell recordings of active cells showing spontaneous action potentials (left) and inactive cells with no action potentials (right). Bar graph representing the ratio of active and inactive cells recorded in PVA from KO and CR mice. This ratio is significantly smaller in KO than in CR mice (*, P < 0.05). B. Threshold measured from first spike of depolarization in KO and CR mice. The threshold was significantly depolarized in KO mice. C. Upper: Example trace of action potential induced by a 1 s, 10 and 20pA current injection steps, while holding membrane potential at −70 mV. Lower: Scatter plot graph showing that the number of spikes induced by 10pA and 20pA current injection at −70 mV was reduced in the KO mice (gray) relative to control mice (black). D. Upper: Example traces of rebound spikes triggered by injection of negative current steps (−50, −100 and −150pA) from a holding potential of −70 mV. Lower—left: The latency to the peak of calcium spike after current injection is significantly longer in Shox2 KO neurons than in control neurons. Lower—right: The areas under voltage traces (between spike traces and −70 mV) indicating plateau depolarizations are significantly larger in Shox2 KO neurons (arrow) than neurons from control mice (*, P < 0.05, ***; P < 0.001).