Figure 3. Overexpression of ST8Sia6 in pancreatic beta cells increases ligands for Siglec-E.

(A) Schematic of gene expression system. In cre-expressing cells, tTA and thus ST8Sia6 expression is induced. (B) Immunofluorescent images of islets stained with anti-insulin (green) and anti-myc-tag (red) antibodies, with DAPI (blue) as counterstain. (C) Extent of recombinant Siglec-E bound to beta cells (defined as live, single CD45⁻, autofluorescent/BL1⁺, FSC^hi, SSC^hi cells) from littermate (n = 3) vs. βST (RIP-cre LNL-tTA ST8Sia6) mice (n = 3), relative to human IgG secondary antibody bound. An unpaired T test was performed with error bars representing standard deviation.