Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 11;12:346. doi: 10.1186/s13287-021-02409-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — SG-callus extract and its major component, shikimic acid, induce stemness in human fibroblast and expression of pluripotent stem cell markers. a Bright-field images of HDF after treatment with SG callus extract (500 μg/ml) for 7 days. Scale bar = 500 μm. b RNA expression of OCT4, NANOG, and Sox2 were analyzed by qRT-PCR. It was normalized to 18s RNA (N = 3 in each group, **p < 0.01). c HPLC chromatogram of the SG-callus extract detected at the UV wavelength of 230 nm. The peak (RT = 4.248 min) is assigned to the shikimic acid. The peak (RT = 18.666 min) is assigned to the caffeic acid. The peak (RT = 21.672 min) is assigned to the trans-ferulic acid. d A colony formation assay was performed on naive HDF and HDF treated with SG-callus extract or shikimic acid, caffeic acid, and trans-ferulic acid at 10 days after treatment. Scale bar = 500 μm. Representative pictures are shown. e Relative stained colonies are quantified (N = 3 in each group, *p < 0.05, ***p < 0.001 compared with the control)