Skip to main content
. Author manuscript; available in PMC: 2021 Jun 12.
Published in final edited form as: Cell Rep. 2021 May 25;35(8):109167. doi: 10.1016/j.celrep.2021.109167

Figure 7. Recognition and elimination of infected primary lymphocytes by autologous effector cells.

Figure 7.

Phytohemagglutinin (PHA)-stimulated primary cells were infected with NL4–3, and antibodies were tested for their ability to bind without effectors or to drive the elimination of infected (p24+CD4−) cells by addition autologous PBMCs for 18 h. Antibodies were tested for binding and elimination over a 4-fold dilution series beginning at 25 μg/ml.

(A) Maximum elimination of infected (p24+CD4−) cells by the indicated antibody. Statistical significance is displayed relative to non-specific IgG1, and only adjusted p < 0.05 are displayed.

(B) Correlation of antibody recognition and elimination of infected cells over the dilution series. Each data point represents the mean value across three donors (ADCC) or two ADCC donors (binding) run in duplicate for the indicated antibody at a single concentration.

(C) Correlation of ADCC activity among NL4–3-infected primary target cells with an NL4–3-infected target cell line (CEM-NKr-CCR5; data in Figure 4).

Error bars in (A) indicate the standard error of the mean, and statistical significance for binding and killing was calculated independently using ANOVA with Dunnett’s multiple-comparisons test. A two-tailed Spearman correlation was used in (B) and (C). Significance is indicated as follows: *p ≤ 0.05, **p ≤ 0.01.