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. 2021 Jan 19;19(6):1155–1169. doi: 10.1111/pbi.13536

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© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Phylogenetic analysis, sequence alignment and subcellular localization of GsERD15B protein. (a) Phylogenetic tree of ERD15 proteins. The 1000 bootstrap values are shown on the branches. (b) Sequence alignment of ERD15 proteins. The conserved PAM2, PAE1 and IqQPR domains are indicated by boxes. Both figures are generated using the full‐length amino acid sequences of the 22 proteins that are homologous with GsERD15B, including AtERD15 (Arabidopsis thaliana, AT2G41430), AtERD15L (Arabidopsis thaliana, AT4G14270), BnERD15 (Brassica napus, ADP37978.1), CaERD15 (Capsicum annuum, ABB89735.1), CpERD15T (Carica papaya, evm.TU.supercontig_197.11), CsERD15B (Cucumis sativus, Cucsa.335550), GmERD15A (Glycine max, Glyma04g28560), GsERD15B (Glycine soja, XP_028191281.1), GmERD15C (Glycine max, Glyma02g42860), GmERD15D (Glycine max, Glyma14g05980), MtERD15A (Medicago truncatula, Medtr3g023110), MtERD15B (Medicago truncatula, Medtr5g091120), OsERD15A (Oryza sativa, Q7XXS2), OsERD15B (Oryza sativa, Q7EZY8), OsERD15C (Oryza sativa, Q5W6M4), SlERD15 (Solanum lycopersicum, NP_001234461), VaERD15 (Vitis amurensis, JQ687321), ZmERD15A (Zea mays, GRMZM2G093325), ZmERD15B (Zea mays, GRMZM2G181551), ZmERD15C (Zea mays, GRMZM2G045178), ZmERD15D (Zea mays, GRMZM2G327692) and ZmERD15E (Zea mays, GRMZM2G037189). (c) Confocal microscopy images showing the subcellular localization of GsERD15B in leaf cells of Nicotiana benthamiana. Bars, 50 μm.