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. 2021 Jan 19;19(6):1155–1169. doi: 10.1111/pbi.13536

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© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Transactivation and protein interaction analyses of GsERD15B. (a) Yeast two‐hybrid (Y2H) assay showing that GsERD15B protein has self‐activation activity but GsERD15B‐PAM2 domain does not have self‐activation activity, and GsERD15B interacts with AtPAB2, AtPAB4, AtPAB8. DDO/X‐α‐gal and QDO/X‐α‐gal represent double dropout medium (without Leu/Trp) and quadruple dropout medium (without Ade/Leu/Trp/His), respectively. BD: the GAL4 DNA binding domain in pGBKT7 vector; AD: the GAL4 activating domain in pGADT7 vector. The BD‐Lam + AD‐T is the negative control and BD‐53 + AD‐T is the positive control. (b) Bimolecular Fluorescence Complementation (BiFC) assay showing that GsERD15B interacts with AtPAB2, AtPAB4, AtPAB8, in leaf cells of Nicotiana benthamiana. YFP, yellow fluorescent protein. Scale bar, 50 μm.