Figure 2.

The effects of DHA and EPA on the constitutive and inducible 20S core subunit expression in astrocytes activated with IL-1β. Astrocytes were incubated with DHA and EPA (30 µM) for 24 h, followed by treatment with IL-1β (10 ng/mL) for 16 h. Total mRNA was isolated from cells by Trizol and β1 (A), β5 (B), β1i (C) and β5i (D) subunits expression was determined by quantitative real-time PCR. The ratios of inducible to constitutive proteasome subunits β1i/β1 (E) and β5i/β5 (F) expression were calculated. * p < 0.05, ** p < 0.01, *** p < 0.001.