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. 2021 May 21;22(11):5443. doi: 10.3390/ijms22115443

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Summary of the procedural timeline for Experiment 1, which employed a within-subjects design to determine (1) the dose–response function for A33 effects upon alcohol (EtOH) intake under 3-bottle drinking-in-the-dark (DID) procedures; (2) the effect of 1.0 g/kg A33 on sucrose (SUC) intake under DID procedures; and (3) the effect of 1.0 mg/kg A33 tests for spontaneous and alcohol-induced changes in locomotor activity, rotarod performance and then alcohol-induced sedation using the regain of righting reflex assay.