(A) Boxplot showing relative expression of ARID1A in breast cancer. (B) Total RNAs were extracted from MCF-10A, MDA-MB-231, MDA-MB-453, and SKBR3 cell lines and subjected to RT-qPCR analysis for ARID1A. (C) ARID1A mRNA levels were not changed by METTL8 knockdown. METTL8 and ARID1A mRNA levels were analyzed by RT-qPCR at 24 h and 48 h after the METTL8 knockdown in MDA-MB-231 cells. (D) ARID1A protein expression levels were increased after METTL8 knockdown. Western blots were subjected to detect ARID1A protein expression 24 h and 48 h after METTL8 siRNA transfection in MDA-MB-231 cells and MDA-MB-453 cells. * indicates p < 0.05, ** p < 0.01, and *** p < 0.001, HBC: human breast cancer.