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In vitro characterization of 111In-DOTA-EB-cRGDfK. (A) The labeling efficiency was 98% by radio-TLC analysis. (B) The radio HPLC analysis revealed that the radiochemical purity was 96% with a retention time of 7.2 min. (C) U-87 MG cells were used for cell-binding assays and competed with DOTA-EB-cRGDfK or DOTA-cRGDfK. The IC50 values of DOTA-EB-cRGDfK and DOTA-cRGDfK were 71.7 nM and 35.2 nM, respectively.
