Scheme 21.
Split-and-click approach to modified sgRNAs. Click reaction conditions: (a) MgCl2, fresh ascorbic acid, CuSO4- tris-(3-hydroxypropyltriazolylmethyl)amine, triethylammonium acetate buffer (pH 7): DMSO (1:1), r.t., 1–2 h.
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