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Solid-phase synthesis of 5′ alkyne modified oligonucleotide and CuAAC reaction: (a) alkynyl linker, PvCl, MeCN/Py (3:1), 5 min, r.t.; (b) I2/Py/H2O, 15 min, r.t. or S8, Et3N, BSA, r.t. 30 min; (c) 3.5% DCA in DCM, 2 min, r.t.; (d) HBTU (2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate), NMM (N-Methylmorpholine), DMF, 30 min, r.t.; (e) azide (2 eq.), CuBr·Me2S (2.5 eq.),. DIPEA, (1,25 eq.) tBuOH/H2O/DMSO (1:1:1) r.t. 2 min.; (f) NH3 in MeOH, r.t. 16 h.
