Table 2.

Epitopes identified in GP5 sequences from Costa Rica PRRSV-2 isolates Phylogenetic analysis of ORF5

Isolate from Costa Rica	Start	End	Match	Length of Match	Identity (%)	Epitope ID and Reference fron literature
RA5_Puriscal-SanJose-CR_2019	29	37	SQLQLIYNL	9	88.9	Gp5-SL,Ostrowski et al. (2002), PRRSV2
	141	155	KGKLYRWRSPVIIEK	15	86.7	Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163
	179	192	TPITKVSAEQWGRP	14	85.7	Gp5-TP, de Lima et al. (2006), PRRSV 2. aa 187–200
	44	53	GTEWLAGKFD	10	80.0	Epitope C Guo et al. 2019, 52-61aa
C4_Corredores-Puntarenas-CR_2019	37	45	SQLQLIYNL	9	88.9	Gp5-SL,Ostrowski et al. (2002), PRRSV2
	117	131	LAAFICFIIRAAKNC	15	80.0	Gp5-LC, Vashisht et al. (2008), PRRSV2, aa 117–131
	149	163	KGRLYRWRSPVIIEK	15	93.3	Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163
	187	200	TPITKVSAEQWGRP	14	85.7	Gp5-TP, de Lima et al. (2006), PRRSV 2. aa 187–200
	52	61	GTDWLAGKFD	10	90.0	Epitope C Guo et al. 2019, 52-61aa
D8_SanRamon-Alajuela-CR_2019	37	45	SQLQLIYNL	9	88.9	Gp5-SL,Ostrowski et al. (2002), PRRSV2
	149	163	KGKLYRWRSPVIIEK	15	86.7	Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163
	187	200	TPITKVSAEQWGRP	14	85.7	Gp5-TP, de Lima et al. (2006), PRRSV 2. aa 187–200
	52	61	GTDWLAGKFD	10	90.0	Epitope C Guo et al. 2019, 52-61aa
R2_SantaBarbara-Heredia-CR_2019	37	45	SHLQLIYNL	9	100.0	Gp5-SL,Ostrowski et al. (2002), PRRSV2
	117	131	LAALTCFTIRFAKNC	15	80.0	Gp5-LC, Vashisht et al. (2008), PRRSV2, aa 117–131
	149	163	KGRLYRWRSPVIIEK	15	93.3	Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163
	187	200	TPVTRVSAEQWGRP	14	92.9	Gp5-TP, de Lima et al. (2006), PRRSV 2. aa 187–200
	27	30	VLVN	4	100.0	Epitope A, Guo et al. 2019, 27-30 aa
	52	61	GTDWLAGKFD	10	90.0	Epitope C Guo et al. 2019, 52-61aa
	37	45	SHLQLIYNL	9	88.9	Epitope B, Guo et al. 2019, 37-45aa
PA3_Coris-Cartago-CR_2019 liz	22	36	KGRLYRWRSPVIIEK	15	93.3	Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163
D7_SanRamon-Alajuela-CR_2019	105	119	KGKLYRWRSPVIIEK	15	86.7	Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163
	143	156	TPITKVSAEQWGRP	14	85.7	Gp5-TP, de Lima et al. (2006), PRRSV 2. aa 187–200
	8	17	GTDWLAGKFD	10	90.0	Epitope C Guo et al. 2019, 52-61aa
RA7_Puriscal-SanJose-CR_2019	37	45	SQLQLIYNL	9	88.9	Gp5-SL,Ostrowski et al. (2002), PRRSV2
	149	163	KGKLYRWRSPVIIEK	15	86.7	Gp5-KK, Vashisht et al. (2008), PRRSV2, aa149–163
	52	61	GTEWLAGKFD	10	80.0	Epitope C Guo et al. 2019, 52-61aa