Figure 4.

Austalide K increased osteoblast differentiation. (A) Austalide K stimulated BMP-2-induced (200 ng/mL) ALP expression for 4 days; (B) Relative ALP mRNA expression levels were evaluated by qRT-PCR; (C) Cytotoxicity of austalide K in C2C12 cells was measured using a CCK-8 kit. C2C12 cells were plated on 96-well plates at 8 × 10³ cells/well and treated with austalide K for 4 days. NC (negative control) and PC (positive control) were BMP-2-untreated and -treated C2C12 cells, respectively. *** p < 0.001.