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Austalide K increased osteoblast-differentiation-related genes. Relative Runx2, OCN, and OPN mRNA expression levels were evaluated by qRT-PCR after 4 days. C2C12 cells were plated in 6-well plates at 8 × 104 cells/well and were treated with austalide K for 6 days. * p < 0.05; ** p < 0.01; *** p < 0.001.
