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PSMA-positive LNCaP, C4-2B, and 22Rv1 cells and PSMA-negative PC-3 cells were incubated with (A) JVM-PE24X7 or (B) PE24X7 for 72 h or (C) preincubated with 1.0 μM JVM for 1 h before JVM-PE24X7 was added. Then, the cell viabilities were determined by MTT assay. (D) EC50 data were calculated with GraphPad Prism. Error bars, means ± SEM (n = 5).
