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. 2021 May 24;13(11):2570. doi: 10.3390/cancers13112570

Figure 2.

Figure 2

Inhibition of HR resensitized the TMZ-resistant U251 cells harboring high HR to TMZ. (A) Quantitative data from immunofluorescence analysis of Rad51 foci in U251, U251TMZR#3, and U251TMZR#8 cells 3 and 7 days after TMZ exposure (100 μM, 3 h). (B) Homologous recombination activity as determined by an in vivo plasmid-based recombination reporter assay. Value of the U251 cells was set at 1. (C) Western blot analysis of RAD51 and β-actin levels in U251TMZR#3 and U251TMZR#8 cells three days following exposure to scramble or RAD51-targeting siRNA. (D) Fluorescence-activated cell sorting analysis of cell cycle distribution in control and RAD51-suppressed U251TMZR#3 and #8 cells 3 and 5 days after TMZ exposure (100 μM, 3 h). (E) Western blot analysis of cleaved PARP, C-leaved caspase 3, and β-actin levels in U251 parental, U251TMZR#3, and U251TMZR#8 cells. (F) Colony formation efficiency of control and RAD51-suppressed U251TMZR#3 and #8 cells following TMZ exposure (0–100 μM, 3 h)., * p < 0.05.