Table 1.
Frequency evolution of alkB and FTO canonical (CAN) and indel (INS, DEL) clones during panning as derived from the Illumina sequencing output.
| alkB | FTO | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Library | Total Paired Reads | CAN (%) |
INS9 (%) |
Total Paired Reads | CAN (%) |
INS25 (%) |
INS27 (%) |
INS1 (%) |
DEL2 (%) |
| Naive | 761,541 | 77.09 | 17.22 | 115,191 | 67.69 | 1.06 | 0.14 | 0.01 | 0 |
| Round 1 | 151,953 | 82.26 | 14.14 | 106,962 | 28.59 | 19.88 | 0.61 | 0.76 | 0.06 |
| Round 2 | 140,577 | 86.26 | 3.71 | 64,156 | 16.7 | 25.24 | 0.71 | 2.6 | 1.67 |
| Round 4 | 128,364 | 85.79 | 4.28 | 9453 | 5.74 | 28.49 | 0.51 | 8.05 | 7.39 |
The first alkB and FTO column represents the total number of paired reads which remained after raw read processing and removal of non-specific reads. The subsequent columns show the fractions (%) of canonical (CAN) and indel clones in each selection round.