Figure 5.

Quantitation of oxidized CL species in isolated pancreatic islets. Islets isolated from 4-month-old Tg and NTg mice were used to quantitate oxidized CL species by (A) tandem mass spectrometry and (B) expressed as a fraction of each individual CL species. Peroxidized CL species in islets were quantitated by (C) tandem mass spectrometry and (D) expressed as a fraction of each individual CL species. Values are means ± SEMs (n = 2-4). (E) Representative immunofluorescent (40×) images of 4-HNE–stained pancreas sections (n = 3-4). *P < 0.05 compared with NTg mice fed the same diet. 4-HNE, 4-hydroxynonenal; CL, cardiolipin; NTg, nontransgenic; Tg, transgenic.