Figure 4.

Increasing concentrations of humanized anti-MUC1 antibodies do not further enhance NK cell degranulation and cytotoxicity. (A) Experimental overview of the analysis of degranulation and cytotoxicity after co-culture of human NK cells with Jurkat MUC-Tn positive tumor cells in the presence of anti-MUC1 antibodies. (B) Fraction of degranulation NK cells after 4 h of co-culture with different effector/tumor ratios and antibody concentrations. (C) Quantification of antibody-dependent cellular cytotoxicity at different E:T ratios and antibody concentrations. Bars indicate mean ± SD, and dots are individual NK cell donors from independent experiments (n = 6 per experimental group). Statistical analysis using two-way ANOVA plus Tukey’s multiple comparisons test. p < 0.05 (*); p < 0.01 (**); p < 0.001 (***).