Fig. 7.

Metastasis prevention via the DLG matrix-enabled sequential delivery strategy. a Schematic illustration of the animal experimental design for inhibition of lung metastasis. b Representative photographs of lung tissues with tumor metastasis. c H&E staining of lung tissues collected from mice in different groups at Day 51 after surgery. Scale bars: 2 mm (top) and 400 μm (bottom). d Quantification of pulmonary metastasis nodules in different groups of 4T1 tumor-bearing BALB/c mice. Data are shown as mean ± SEM (n = 4). e, f Proportions of T_EM cells and T_CM cells in lymph nodes (gated on CD3⁺ CD8⁺ T cells) examined after 1 day post-intravenous infusion of the 4T1 cells. Data are shown as mean ± SEM (n = 3). The comparison of two groups was followed by student’s t test (two-tailed). *p < 0.05, **p < 0.01 and ***p < 0.001