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. 2021 Apr 8;34(4):1114–1122. doi: 10.1007/s13577-021-00527-x

Fig. 3.

Fig. 3

Down-regulation of LINGO-1 increased cell viability and decreased apoptosis in OGD-induced HT22 cells. a LINGO-1 mRNA expression was detected by qPCR, and b WB was used to assay LINGO-1 protein expression. b Cell viability of HT22 cells during OGD/R treated with sh-LINGO-1 was measured by CCK8. c Apoptosis was examined by flow cytometric analysis using Annexin V/PI assay. d Expression of apoptosis-related proteins Bax, Bcl-2, Caspase 3, and Cleaved caspase 3 were detected by western blot. Data are expressed as mean ± SD and *p < 0.05; **p < 0.01; ***p < 0.001