Fig. 4.

UV mutagenesis of JR11.1 results in mutants that show ferulic acid independent expression of amdS and lux. Spores of reporter strain JR11.1 were exposed to a series of UV light doses, and the survival rate after each dose was determined. a Spores with a survival rate of ± 60 % were plated out on selective medium (1 % D-fructose + 10 mM acetamide). b Mutants that were able to grow were purified on selective medium. c and d Expression of the second reporter gene lux was measured in a lux assay, using D-fructose as carbon-source without (c) or with the addition of ferulic acid (FA) (D). Growth and luciferase activity were measured every 15 min for 24 h at 30 °C