Fig. 5.

δ-TT induces mitochondrial ROS overproduction in human melanoma cells. a A375 and BLM cells were treated with 15 µg/mL δ-TT for 12 h and then stained with MitoSOX Red (5 µM, 10 min) fluorescent probe. ROS generation in mitochondria was assessed by flow cytometry. T-test. Mean values ± SEM are shown. ***P < 0.001 versus controls. b A375 and BLM cells were incubated with 5 mM NAC (N-acetyl-l-cysteine), the ROS scavenger, for 2 h and then treated with 15 μg/mL δ-TT for 24 h. MTT assay was performed to assess melanoma cell viability. Three experiments have been performed. One-way analysis of variance. Post-test: Bonferroni’s test. Mean values  ±  SEM are shown. **P < 0.01 versus controls