Fig. 7.

δ-TT causes Ca²⁺ homeostasis dysregulation in human melanoma cells. a A375 and BLM cells were treated with 15 μg/mL δ-TT for 12 h and then stained with 5 µM Fluo-3 AM for 30 min. Cytoplasmic Ca²⁺ levels were assessed by flow cytometry. Three experiments have been performed. T-test. Mean values  ±  SEM are shown. *P < 0.05 versus controls; ***P < 0.001 versus controls. b A375 and BLM cells were treated with 15 μg/mL δ-TT for 12 h and then stained with 5 µM Rhod-2 AM for 30 min. Mitochondrial Ca²⁺ levels were evaluated by flow cytometry. Three experiments have been performed. T-test. Mean values  ±  SEM are shown. *P < 0.05 versus controls; ***P < 0.001 versus controls. c A375 and BLM cells were incubated with the IP3R inhibitor 2APB (20 µM, 2 h) and then treated with 15 μg/mL δ-TT for 24 h. MTT assay was performed to evaluate cell viability. Three experiments have been performed. Mean values  ±  SEM are shown. One-way analysis of variance. Post-test: Bonferroni’s test. *P < 0.05 versus controls; **P < 0.01 versus controls. d A375 and BLM cells were incubated with the VDAC blocker DIDS (75 µM, 2 h) and then treated with 15 μg/mL δ-TT for 24 h. MTT assay was performed to evaluate cell viability. Three experiments have been performed. Mean values  ±  SEM are shown. One-way analysis of variance. Post-test: Bonferroni’s test. **P < 0.01 versus controls; ***P < 0.001 versus controls