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20 individuals were age- and sex-matched to form 10 AD case-control pairs. CSF samples were collected by lumbar puncture, after which proteins were extracted, denatured, and digested before being analyzed by tandem mass spectrometry. Three different analysis strategies were utilized: single-shot (orange), addition of FAIMS (yellow), and single-shot analyses run in parallel with a fractionated commercial CSF sample (green).
