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. 2021 Jun 12;12(6):609. doi: 10.1038/s41419-021-03895-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Heat map of whole-gene transcriptomic changes detected by RNA-seq in HepG2 cells that knockdown H2A.Z expression(Software: R pheatmap, R package). b Volcanogram of changes at the whole-gene transcriptomic level in HepG2 cells that knockdown H2A.Z expression(Software: ggplot2, R package). c–f Enrichment of pathway in GSEA analysis of genes altered as described above (Software: GESA). g Venn diagram depicting the overlapping variation genes in RNA-seq and Chip-seq of H2A.Z(Software: Venn Diagram, R package). h KEGG pathway analysis was used to analyze the combined analysis results showed that H2A.Z might be associated with transcriptional misregulation in cancer signaling pathway in HCC cells (Software: ggplot2, R package). i IGV diagram of the downstream target gene of H2A.Z in the combined analysis data. j, k Changes in the expression of target genes upon H2A.Z were knockdown in HCC cells detected by qPCR and western blot. l Immunohistochemical analysis of target proteins in tumors in nude mice from different groups(Scale bars: 20 μm). The data are expressed in terms of mean ± SD (Student’s t-test used in Fig k; **P < 0.01; ***P < 0.001. ****P < 0.0001; n = 3 in each group).