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. 2021 Jun 12;12(6):609. doi: 10.1038/s41419-021-03895-2

Fig. 4. BCL6 is the main interaction protein of H2A.Z in HCC, and the protein domains binding to H2A.Z are BTB and PHA03247.

Fig. 4

a Motif analysis of H2A.Z and BCL6 in chip-seq of H2A.Z. b Co-IP analysis of H2A.Z and BCL6 in HepG2 cell. c IGV diagram of the downstream target gene of H2A.Z in the combined analysis data of H2A.Z RNA-seq and BCL6 chip-seq. d Validation of BCL6 gene knockout effect detected by western blot. e, f QRT-PCR, and western blot verified the changes of downstream target genes after BCL6 knockdown in H2A.Z overexpressed HCC cell lines. g Schematic diagram of the BCL6 protein domain(https://www.uniprot.org/). h The interaction of BCL6 protein regions with H2A.Z in HEK293T cells was confirmed by a Co-IP assay, which showed the protein domains binding to H2A.Z are BTB and PHA03247. i, j Transwell assay was adopted to test cell migration and invasion under different contexts. The data are expressed in terms of mean ± SD (Student’s t-test used in Fig h, i and j; **P < 0.01; ***P < 0.001; ****P < 0.0001; n = 3 in each group).