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. 2021 Jun 12;12(6):609. doi: 10.1038/s41419-021-03895-2

Fig. 8. Schematic diagram of the action mechanism of H2A.Z in HCC.

Fig. 8

H2A.Z acetylation by lincZNF337-AS1 via KAT5 implicated in the transcriptional misregulation in cancer signaling pathway in HCC. Fig.S1 Bioinformatics data analysis. a–b Heat maps and volcanograms of 23 pairs of HCC and paired paracancer tissues analyzed using RNA-seq in the GEO database(Software: R pheatmap and ggplot2, R package). c–d Heat maps and volcanograms of the three pairs of HCC and paired paracancer tissues analyzed by RNA-seq(Software: R pheatmap and ggplot2, R package). e Graphical comparison of the two groups of gene transcriptome data analyzed using RNA-seq, smooth curve, indicating high consistency of data(Software: ggplot2, R package) (Fig. S2) Flow cytometry analysis was conducted for detecting the cell cycle. The data are expressed in terms of mean ± SD (Student’s t-test; *P < 0.05; **P < 0.01; n = 3 in each group) (Fig. S3) Verification of downstream target genes of H2A.Z. a Schematic diagram of transcriptional misregulation in the cancer signaling pathway, E47 synonymsTCF3; p21 synonyms CDKN1A; PFTK1 synonyms CDK14; plakoglobin synonyms JUP. b Chip-qPCR assay identifies the downstream target genes of H2A.Z. The data are expressed in terms of mean ± SD (Student’s t-test; ***P < 0.001; ****P < 0.0001; n = 3 in each group) (Fig. S4) Verification of downstream target genes of BCL6 and knockdown identification of lincZNF337-AS1 in vitro. a Chip-qPCR assay identifies the downstream target genes of H2A.Z. b–e Identification of lincZNF337-AS1 gene knockdown effect by qRT-PCR and ISH in HCC cell lines and H2A.Z overexpression HCC cell lines(Scale bars: 20 μm). The data are expressed in terms of mean ± SD (Student’s t-test; ***P < 0.001; ****P < 0.0001; n = 3 in each group) (Fig. S5) analysis of the in vitro transcription products. a Electrophoretic images of PCR products after plasmid amplification, the Maker strip size from top to bottom is in order: 5 K, 3 K, 2k, 1.5 K, 1 K, 750 bp, 500 bp, 250 bp, 100 bp. b Electrophoretogram of transcription products in vitro, Maker strip size from top to bottom: 2k 1 K 750 bp 500 bp 250 bp 100 bp (n = 3 in each group).