Figure 3.

Effect of exogenous H₂O₂ on root gravitropic response. (A) Seedlings were grown vertically for 5 days, then they were transferred to media containing different H₂O₂ concentrations and immediately rotated by −135° for 24 h. Red arrows indicate the direction of the gravity vector. The pictogram at the lower right corner shows the mode of the determination of the root curvature degree, the red semicircle with arrows indicates the measured angle. (B) Quantitative analysis of the H₂O₂ effect on the degree of gravitropic curvatures of wild type (AtCol-0) and mutant (Atcrk5-1) roots. Asterisks indicate significant differences at ** p < 0.01 or *** p < 0.001 between the wild type (Col-0) and the mutant (Atcrk5-1). Measurements were performed with at least 30 seedlings per each line. Bars indicate standard error. At least three biologically independent experiments were carried out with the same statistical results.