Figure 2.

MIN6 cells viability after 24-h (A,B,E) and 48-h (C,D,F) treatment with 1-GERA-LPC, 1-GA-LPC, 1-CA-LPC, 1-FARA-LPC and corresponding free terpene acids (GERA, GA, CA, FARA). Cytotoxicity was assessed based on the activity of mitochondrial reductases with the PrestoBlue^TM Cell Viability Reagent (A–D) in the range of 5–500 μM concentrations (A–D). The lowest compound concentrations (5–25 μM) were re-assessed in cytotoxicity experiments referring to the number of living cells to the quantity of DNA with the CyQUANT^TM Cell Proliferation Test (E,F). The results are expressed as % of living cells after treatment with respective compounds solvent (DMSO:EtOH) and presented as means ± SEM from 3-4 independent experiments, 3-6 biological replicates each. * p < 0.05 is considered as significantly different from DMSO:EtOH.