Figure 4.

Intracellular Ca²⁺ mobilization in MIN6 cells triggered by 1-GERA-LPC, 1-GA-LPC, 1-CA-LPC, 1-FARA-LPC and corresponding acids (GERA, GA, CA, FARA) versus the compound solvent (DMSO:EtOH) at 2 mM (A–C) and 20 mM glucose conditions (D–F), with simultaneous monitoring of membrane integrity via PI incorporation (G–I)—data were collected from both 2 and 20 mM glucose conditions). The results are presented as the real-time kinetics of [Ca²⁺]_i and [PI] changes inside the cell during 3 min-time monitoring as well as AUC ± SEM from 3-4 independent experiments, 3-5 biological replicates each. The compounds were tested at 10 μM concentration. p < 0.05 is considered as significantly different from DMSO:EtOH at 2 mM (^) or 20 mM (*) glucose conditions.