Figure 7.

GLUTag cells viability after 24 h (A,C) and 48 h (B,D) treatment in fasting conditions with 1-GA-LPC and 1-CA-LPC and their corresponding acids (GA, CA). Cytotoxicity was assessed in the range of 5–25 μM concentrations, based on the activity of mitochondrial reductases with the PrestoBlue^TM Cell Viability Reagent (A,B), and referring the number of living cells to the quantity of DNA with the CyQUANT^TM Cell Proliferation Test (C,D). The results are presented as means ± SEM from 3-4 independent experiments, 3-6 biological replicates each, and expressed as % of living cells after treatment with respective compounds solvent (DMSO:EtOH). ^ p < 0.05 is considered as significantly different from DMSO:EtOH.