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. 2021 May 26;22(11):5639. doi: 10.3390/ijms22115639
C control
CD circular dichroism
CFE cell free extract
DIR dirigent protein
DLS dynamic light scattering
DSF differential scanning fluorimetry
DTT dithiothreitol
F# SEC fraction
FT flow through
GFP green fluorescent protein
GST glutathione S-transferase-tag
IMAC immobilized metal-chelate affinity chromatography
JRL jacalin-related lectin
JRL + DIR stoichiometric mixture of JRL and DIR domain
gJRL galactose-specific jacalin-related lectins
mJRL mannose-specific jacalin-related lectins
M marker
OsJAC1_P1 main OsJAC1 peak of SEC
OsJAC1_P2 secondary OsJAC1 peak of SEC
PMSF phenylmethylsulfonyl fluoride
RE raw extract/disrupted cell suspension
RT room temperature
SEC size-exclusion chromatography
Tm melting point
W1 wash fraction with 100% buffer A (20 mm imidazol)
WF wash fraction with 30% buffer B (250 mm imidazol)