Table 1.
Composition of growth medium for human and mouse organoid cultures.
| Reagent | Function | Human | Mouse | |
|---|---|---|---|---|
| Normal | Tumor | Normal | ||
| Advanced DMEM/F12 | Basal medium | + | + | + |
| HEPES | pH buffer | + | + | + |
| Glutamax | L-glutamine supplier | + | + | + |
| B27 supplement | Vitamins/cofactors mix | + | + | + |
| N2 supplement * | Vitamins/cofactors mix | +/− | +/− | − |
| N-acetyl-L-cysteine | Antioxidant | + | + | + |
| Antibiotics | To avoid bacterial contamination | + | + | + |
| Wnt3A-cond. medium | Stemness/Wnt signaling activator | + | + #/− | + |
| RSPO | Stemness/Wnt signaling enhancer | + | + #/− | + |
| Nicotinamide * | Stemness/cystic phenotype | +/− | +/− | − |
| PGE2 * | Wnt activator/cystic phenotype | +/− | +/− | − |
| EGF | Mitogen | + | + | + |
| Gastrin | Mitogen | + | + | − |
| FGF-2 | Mitogen | + ¥/− | + ¥/− | − |
| IGF-1 | Mitogen | + ¥/− | + ¥/− | − |
| Noggin | BMP inhibitor | + | + | + |
| Y-27632 † | ROCK/anoikis inhibitor | + | + | + |
| SB202190 | p38MAPK inhibitor | +/− ¥ | + #/− ¥ | − |
| A 83-01/LY2157299 | TGF-β inhibitor | + | + | − |