Table A4.
Summary of fish behavioral endpoints measured in each behavioral test during this experiment.
| Index | Behavior Endpoints (Units) | Definition | Applied to |
|---|---|---|---|
| 1-1-1 & 1-2-1 | Average speed (cm s−1) | Total distance traveled by fish divided by total time duration | Novel Tank Test (1-1 for 0–11 min and 1-2 for 15–31 min) |
| 1-1-2 & 1-2-2 | Freezing time movement ratio (%) | Total percentage of time when fish’s speed less than 1 cm s−1 | |
| 1-1-3 & 1-2-3 | Swimming time movement ratio (%) | Total percentage of time when fish’s speed is between 1 and 10 cm s−1 | |
| 1-1-4 & 1-2-4 | Rapid movement ratio (%) | Total percentage of time when fish’s speed more than 10 cm s−1 | |
| 1-1-5 & 1-2-5 | Time in top duration (%) | Total time spent in the top portion of the novel tank in percentage | |
| 1-1-6 & 1-2-6 | Number of entries to the top | Total times fish enter the upper half of the tank | |
| 1-1-7 & 1-2-7 | Latency to enter the top (s) | The amount of time it takes the fish to cross into the upper half of the tank | |
| 1-1-8 & 1-2-8 | Total distance traveled in the top (cm) | Total distance traveled in the top portion of the novel tank | |
| 1-1-9 & 1-2-9 | Thigmotaxis (cm) | The average distance of the fish from the center of the tank | |
| 2-1 | Mirror biting time percentage (%) | Total percentage of time when fish stayed in the mirror biting zone | Mirror Biting Test |
| 2-2 | Longest duration in the mirror side percentage (%) | Total percentage of fish longest duration stayed in the mirror biting zone | |
| 3-1 | Approaching predator time percentage (%) | Total percentage of time when fish stayed in the approaching predator zone | Predator Avoidance Test |
| 3-2 | Average distance to the predator’s separator (cm) | Average distance of fish to the predator’s separator | |
| 4-1 | Conspecific interaction time percentage (%) | Total percentage of time when fish stayed in the conspecific interaction zone | Social Interaction Test |
| 4-2 | Average distance to the conspecific’s separator (cm) | Average distance of fish to the conspecific’s separator | |
| 4-3 | Longest conspecific interaction percentage (%) | Total percentage of fish longest duration stayed in the conspecific interaction | |
| 5-1 | Average inter-fish distance (cm) | Average distance between the body center of every member of the shoal | Shoaling Test |
| 5-2 | Average shoal area (cm2) | Average size of the shoal | |
| 5-3 | Average nearest neighbor distance (cm) | Distance for the body center of each fish to the closest neighboring fish | |
| 5-4 | Average farthest neighbor distance (cm) | Distance for the body center of each fish to the farthest neighboring fish | |
| 6-1-1 & 6-2-1 | Average speed (cm s−1) | Total distance traveled by fish divided by total time duration | Circadian Locomotor Activity Rhythm (6-1 for day cycle and 6-2 for night cycle) |
| 6-1-2 & 6-2-2 | Average Angular Velocity (°/s) | Total turning angle of fish divided by total time duration | |
| 6-1-3 & 6-2-3 | Meandering (°/m) | Total turning angle of fish divided by total distance traveled by fish | |
| 6-1-4 & 6-2-4 | Freezing time movement ratio (%) | Total percentage of time when fish’s speed less than 1 cm s−1 | |
| 6-1-5 & 6-2-5 | Swimming time movement ratio (%) | Total percentage of time when fish’s speed is between 1 and 10 cm s−1 | |
| 6-1-6 & 6-2-6 | Rapid movement ratio (%) | Total percentage of time when fish’s speed more than 10 cm s−1 |