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. 2021 May 30;22(11):5874. doi: 10.3390/ijms22115874

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Depletion of Sdc-1 has a negative effect on the stem cell phenotype (CD44⁺/CD24^-/low) of MCF-7 and MDA-MB-231 cells. (A,D) The percentages of CD24-/low/CD44^-/low, CD24^-/low/CD44high, CD24high/CD44^-/low and CD24high/CD44high populations were measured by flow cytometry post-transfection and 96 h after 50 μg/mL HA treatment. Representative density plots are shown. (B–C,E–F) Mean intensity of fluorescence (MIF) of CD24 (B,E) and CD44 (C,F) was determined by flow cytometry and plotted as a fold of C/M (n = 5 MCF-7, n = 3 MDA-MB-231). Data represent the mean ± SEM. Asterisks on the top of the bars indicate a significant difference between C/M and all other conditions. The line over the bars indicates a significant difference between C/HA and KD/M and KD/HA (* p < 0.05, ** p < 0.01, *** p < 0.001). C/M: control; C/HA: control plus hyaluronic acid treatment; KD/M: Sdc-1 knockdown; KD/HA: Sdc-1 knockdown plus hyaluronic acid treatment.