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. 2021 May 30;22(11):5874. doi: 10.3390/ijms22115874

Figure 4.

Figure 4

Sdc-1 depletion promotes cell cycle progression in MCF-7 cells and increases MSI-2 and MYC expression in MDA-MB-231. (A–D,F–I) Expression of CCND1 (A,F), CCNB1 (B,G), MSI2 (C,H), and MYC (D,I) was analyzed post-transfection and 24 h after 50 μg/mL HA treatment by RT-qPCR, normalized to β-ACTIN expression, and shown as fold of C/M (n = 3 MCF-7, n = 4 MDA MB-231). Data represent the mean ± SEM. (E,J) Cell cycle phase composition was determined by DAPI staining and flow cytometry after Sdc-1 depletion and 96 h of 50 μg/mL HA treatment (n = 3 MCF-7, n = 4 MDA-MB-231). Asterisks on the top of the bars indicate a significant difference between C/M and all other conditions. The line over the bars indicates a significant difference between C/HA and KD/M and KD/HA (* p < 0.05, *** p < 0.001). C/M: control; C/HA: control plus hyaluronic acid treatment; KD/M: Sdc-1 knockdown; KD/HA: Sdc-1 knockdown plus hyaluronic acid treatment.