Skip to main content
. 2021 May 31;22(11):5942. doi: 10.3390/ijms22115942

Figure 5.

Figure 5

Relative expressions of MdPRPs in response to various stresses. (A) Heat treatment: treatment at 48 °C for 6 h, and the heat stress-treated leaves were collected at 0, 1, 2, 4, 5, 6 h; (B) cold stress: treatment at 4 °C for 24 h, and the cold-treated leaves were collected at 0, 2, 4, 8, 12, 24 h; for (C) drought stress, (D) salt stress, and (E) ABA stress, PEG6000 (10%; w/v), 150 mM NaCl, or 100 mM ABA were added, respectively, and the leaves were collected at 0, 2, 4, 8, 12, 24 h. MdMDH primers were used as the reference gene for each target gene. Transcript level at the 0 h in every treatment for each gene was taken as 1 when calculating relative expression levels, and those at other points were accordingly normalized and presented as relative fold changes. One-way ANOVA was used to compare statistical difference based on Tukey’s multiple range tests at significance levels of p < 0.05. Each sample was analyzed in standard deviation of three biological replicates and three technical replicates in each biological sample.