Table 1.
DPPH radical scavenging and anticholinesterase activities of the methanol extract and isolated compounds.
| Compounds | DPPH Radical Scavenging | Anticholinesterase | ||
|---|---|---|---|---|
| % Inhibition | IC50 (µM) | % Inhibition | IC50 (µM) | |
| Hexane extract | 7.2 ± 2.33 1 | ND | ND 4 | ND |
| Ethyl acetate extract | 68.68 ± 1.42 1 | ND | ND 4 | ND |
| Methanol extract | 68.72 ± 0.32 1 | ND | 61.90 ± 0.08 3 | ND |
| Betulinic acid (1) | 15.90 ± 0.87 2 | ND | 39.95 ± 1.53 2,5 | ND |
| Ursolic acid (2) | 12.69 ± 1.33 2 | ND | 73.39 ± 1.32 2,5 | 159.32 ± 1.34 5 |
| Jacoumaric acid (3) | 19.40 ± 2.51 2 | ND | 29.73 ± 3.12 2,5 | ND |
| Corosolic acid (4) | 3.61 ± 0.63 2 | ND | 11.18 ± 0.30 2,6 | ND |
| Daucosterol (5) | 4.11 ± 0.81 2 | ND | 5.53 ± 1.47 2,6 | ND |
| Gallic acid (6) | 95.80 ± 0.32 2 | 14.46 ± 0.29 | 81.64 ± 0.29 2,6 | 161.06 ± 9.52 6 |
| Myricitrin (7) | 95.27 ± 0.45 2 | 31.01 ± 0.90 | 10.91 ± 1.69 2,6 | ND |
| Quercitrin (8) | 93.16 ± 0.11 2 | 38.34 ± 0.74 | 10.21 ± 1.26 2,6 | ND |
| Ascorbic acid (Vitamin C) | ND | 59.73 ± 2.73 | ND | ND |
| Trolox | ND | 50.10 ± 3.56 | ND | ND |
| Galantamine 5 | ND | ND | ND | 14.76 ± 0.63 5 |
| Galantamine 6 | ND | ND | ND | 6.02 ± 0.42 6 |
ND = not determine; 1 at conc. 50 µg/mL, 2 at conc. 100 µg/mL, 3 at conc. 300 µg/mL, 4 solubility problem, 5 dissolved with 50% DMSO–20% Tween 20 in buffer, 6 dissolved with 50% methanol in buffer.