Figure 7.

Cell−cell interaction between naïve TH cells and CD4 CTL prevents the proliferation-inducing effects of the CD4 CTL secretome. (a) Representative plot and cumulative data of proliferation of naïve TH cells when co-cultured with Tregs, or with CD4 CTL, or with Tregs and CD4 CTL. The control (dotted line) indicates the proliferation of naïve TH cells when cultured without Tregs and CD4 CTL, which is also displayed in the uppermost panel of the representative plot. Error bars indicate mean ± SEM for n = 7 donors from seven independent experiments; data analysed with repeated measures one-way ANOVA, post hoc Tukey. (b) Proliferation of naïve TH cells when cultured alone or co-cultured in a transwell assay with TH cells placed in the bottom well and CD4 CTL in the upper well. Representative flow cytometry plot and cumulative data. Error bars indicate mean ± SEM for n = 3 donors from two independent experiments; data analyzed with paired t-test.