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. 2021 May 25;22(11):5602. doi: 10.3390/ijms22115602

Figure 4.

Figure 4

DON delays growth factor-induced cell cycle progression and inhibits proliferation and migration of VSMCs. (A,B) Representative western blots showing the effects of DON on the level of cyclin D1 in VSMCs stimulated with FBS (A) and PDGF (B). Data in bar graphs are mean ± SEM of three independent measurements. (C,D) Histogram showing the cell cycle distribution of VSMCs stimulated with FBS (C) and PDGF (D). (E,F) Effects of DON on proliferation of VSMCs stimulated with FBS (E) and PDGF (F). (G,H) Representative western blots showing the effects of DON on the level of PCNA in VSMCs stimulated with FBS (G) and PDGF (H). Data in bar graphs are mean ± SEM of three independent measurements. (I,J) Transwell migration (upper) and wound healing assays (lower) showing the effects of DON on migration of VSMCs stimulated with FBS (I) and PDGF (J). Data in bar graphs are mean ± SEM of three independent measurements. * p < 0.05, ** p < 0.01, and *** p < 0.001.