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. 2021 May 28;22(11):5766. doi: 10.3390/ijms22115766

Figure 1.

Figure 1

USP29 stabilizes Cdc25A protein expression. (A,B) HEK293 cells were co-transfected with Myc-Cdc25A and wild-type (WT) GFP-USP29 or GFP-USP29 C294A (CA) in increasing concentrations. Western blots were performed with the indicated antibodies. (C) HeLa cells were co-transfected with sgRNAs (sgRNA1 and sgRNA2) targeting USP29 and Cas9, and USP29 knockdown was assessed using Western blot analyses. (D) HEK293 cells were co-transfected with sgRNA1 targeting USP29 and Cas9 along with Myc-Cdc25A. The effect of USP29 knockdown on Myc-Cdc25A was assessed using Western blot analyses. (E) HeLa cells were transfected with sgRNA1 targeting USP29 and Cas9, and the effect of USP29 knockdown on endogenous Cdc25A protein expression was assessed using Western blot analyses. (F) HEK293 cells were transfected with Myc-Cdc25A or co-transfected with sgRNA1 targeting USP29 and/or GFP-USP29, and exogenous expression of Myc-Cdc25A was assessed using Western blotting with the indicated antibodies. (G) HeLa cells were transfected with sgRNA1 targeting USP29 and/or GFP-USP29 to assess endogenous Cdc25A protein expression. Western blots were performed using the indicated antibodies.