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. 2021 May 28;22(11):5766. doi: 10.3390/ijms22115766

Figure 2.

Figure 2

USP29 binds to and co-localizes with Cdc25A. (A,B) HEK293 cells were transfected with the indicated plasmids to investigate the interactions between GFP-USP29 and Myc-Cdc25A. (C,D) HeLa cells were transfected with either GFP-USP29 or Myc-Cdc25A, and the cells were immunostained to assess co-localization of USP29 and Cdc25A. Both USP29 and Cdc25A antibodies are produced by the same species, and thus we transfected either GFP-USP29 or Myc-Cdc25A in HeLa cells and immunostained with GFP and Cdc25A or Myc and USP29-specific antibodies. Scale bar = 20 µm.