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USP29 deubiquitinates and extends the half-life of Cdc25A. (A) HEK293 cells were transfected with the indicated plasmids to analyze the deubiquitinating activity of USP29 on Cdc25A. (B–D) HEK293 cells were transfected with only Myc-Cdc25A (B) and in the presence of USP29 (C) or sgRNA1 targeting USP29 (D) and treated with CHX (100 µg/mL) for the indicated time periods. Western blot analyses were performed with the indicated antibodies to determine the half-life of Cdc25A.
