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. 2021 May 28;22(11):5766. doi: 10.3390/ijms22115766

Figure 4.

Figure 4

USP29 knockdown leads to Cdc25A-mediated cell cycle arrest and induces apoptosis. (A) HeLa cells were transfected with the indicated plasmids, and the status of the cell cycle was determined by the direct measurement of DNA content using flow cytometry after staining with propidium iodide (PI). (B) HeLa cells were transfected with the indicated plasmids and incubated with BrdU to analyze incorporation into the DNA by flow cytometry. (C) HeLa cells were transfected with the indicated plasmids and stained with anti-γH2AX antibody and immunofluorescence analyses were performed. Scale bar = 50 µm. (D) HeLa cells were transfected with the indicated plasmids and treated with or without etoposide (5 µM) for 12 h to identify the sub-G1 cells by flow cytometry. All data points represent the mean ± standard deviation of three independent experiments (** p < 0.01, *** p < 0.001, and **** p < 0.0001).