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. 2021 May 28;22(11):5814. doi: 10.3390/ijms22115814

Figure 2.

Figure 2

Similar UV damage repair kinetics in A2780 cells compared to Acis cells. (A) Representative blot of excised oligos released during NER following 20 J/m2 UVC irradiation in A2780 and Acis cells. (B) Normalized excision products calculated by dividing excision product signal by 50 nt spike- in DNA amounts. Data are represented as mean ± standard error from three biological replicates. P value based on paired Student’s t-test. (C) Representative immuno-slot blot for CPD damage signal at different time points following UV treatment (left panel). Total nucleic acid amounts measured by SYBR-Gold staining (right panel). (D) Damage signal normalized to total nucleic acid amounts, showing CPD repair kinetics of A2780 and Acis cell lines. Averages and error bars (SEM) are shown for data from four biological replicates. (E) Representative immuno-slot blot for 6-4PP damage signal at different time points following UV treatment (left panel). Total nucleic acid amounts measured by SYBR-Gold staining (right panel). (F) Damage signal normalized to total nucleic acid amounts, showing 6-4PP repair kinetics of A2780 and Acis cell lines. Averages and error bars (SEM) are shown for data from five biological replicates. (G) Cell viability of A2780 and Acis cell lines following increasing UV doses.