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Functional amines conjugation to alginate with help of EDC/NHS coupling. (a)-scheme of the reaction; (b) 1H NMR spectrum of sodium alginate measured in D2O at 50 °C (bottom); 1H NMR spectrum of Alg-PAm measured in D2O at 50 °C (middle); 1H NMR spectrum of Alg-N-AEMI measured in D2O at 50 °C (top). Specific signals proving the modification are marked with color.
