Table 2.
Reaction conditions, yields, and substitution degree of the modification for all used methods. The molar ratio between alginate and substitutes was kept at 1 to 0.01 for all the experiments. All values are reported as mean with the standard deviation (n = 3).
| Name | Substitute | Reaction Conditions | Yield, % | SD, Mol% | |
|---|---|---|---|---|---|
| step one | Alg-TYRAY(EDC) | TYRAY | EDC/NHS, MES buffer | 36.5 ± 2.7 | 2.3 ± 1 |
| Alg-TYRAY(DMTMM) | TYRAY | DMTMM, water | 16.7 ± 1.3 | 7.8 ± 2.4 | |
| Alg-TYRAY(DMTMM)*,1 | TYRAY | DMTMM*, water | 68 ± 2 | 4.3 ± 0.5 | |
| Alg-N-AEMI | N-AEMI | DMTMM, water | 79.8 ± 3.2 | 6.1 ± 0.3 | |
| Alg-PAm | PAm | DMTMM, water | 79.1 ± 7 9 | 8.6 ± 1.7 | |
| Alg(d)-Pam 2 | PAm | DMTMM, water | 92.7 ± 1.5 | 7.1 ± 1.4 | |
| step two | Alg-N-AEMI-RGD | RGD | MES buffer | 36.5 ± 1 | 4.1 ± 1.1 |
| Alg-PAm-RDG | N3- RGD | Cu-THPTA, sodium ascorbate, water | 72.5 ± 0.6 | 7.2 ± 0.9 | |
| Alg-PAm-TYRAY | N3- TYRAY | Cu-THPTA, sodium ascorbate, water | 74.4 ± 4.5 | 8.4 ± 0.5 |
1 Deferent sequence of steps: Alginate was mixed first with DMTMM and then with peptide solu-tion. 2 Dialyzed alginate was used.